An Abstract of a Thesis

Field and Laboratory Investigations of Trout Subjected to Fluctuating Water Quality Variables

Stacy M. Xenakis

1996

Master of Science in Biology

In 1995, the Caney Fork River below Center Hill Dam, Tennessee, was stocked with 108,830 harvestable (> 200 mm total length) rainbow trout Oncorhynchus mykiss and 20,949 harvestable brown trout Salmo trutta. Cohorts of both species (N = 3,000-4,000) were microtagged and released into the tailwater on 6 April 1995. An additional cohort of tagged rainbow trout was stocked on 30 June (5,877 fish). From 5 April to 30 September, a stratified roving creel survey collected information on angler use, catch, and harvest of marked and unmarked trout. Fishing pressure in 1995 was estimated at 74,534 h and nearly 10 times as many rainbow trout (41,382) than brown trout (4,210) were harvested, despite the fact that only 4 times as many rainbow trout were stocked. Return rates for tagged cohorts of rainbow trout ranged from 55% to 71%, but only 13% of the tagged brown trout were harvested. The harvest of tagged rainbow trout stocked on 6 April and 30 September approached zero about 140 d and 50 d post-stocking, respectively. In late summer, dissolved oxygen concentrations routinely dropped below 3 mg/L at the onset of generation. Once discharge ceased, dissolved oxygen concentrations slowly increased until generation resumed. Temperatures also decreased from 16 degrees C to 12 degrees C during generation.

Approximately 30 rainbow trout (150-160 mm total length) were placed into each of three 341-L tanks and temperatures were manipulated to simulate three possible temperature regimes present below Center Hill Dam: constant 12 degrees C (control), fluctuations from 12 to 17 degrees C, and fluctuations from 12 to 22 degrees C. A flucuation cycle lasted 5 d and at the end of six full cycles, fish were individually placed into a stamina tunnel. After a habituation period of five min at 7 cm/s, the velocity was abruptly increased to 20cm/s. Fish held at a constant 12 degrees C had the greatest stamina (P=0.05). Instantaneous growth rates varied significantly (P=0.05), and differences in mean total lengths and mean weights at the end of the experiment, absolute growth rates, and relative growth rates were marginally significant (P=0.09-0.13). Fish reared in the 12 to 17 degree C regime grew the slowest, had the lowest stamina, and had the lowest liver somatic index.

  

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